Search results for "Brassica villosa"
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Stress integrated tests and cytological analyses reveal Brassica villosa subsp. drepanensis seed quality decrease upon long-term storage
2016
Under stress integrated germination test (SIGT), seeds undergo osmo-saline stresses, which enable to detect differences in vigour of long-term stored seeds with high germination percentage (G%). The quality of Brassica villosa subsp. drepanensis seeds stored in a genebank (at -20°C for 16 years) was compared with seeds at harvest by standard germination tests (GT), SIGT and cytogenetic analysis. No differences were detected in G% and mean germination time under GT. Conversely, SIGT performed with NaCl -0.9MPa osmotic potential did not influence G% at harvest but reduced that of stored seeds, SIGT at -1.4MPa reduced G% of both. Cytogenetic analysis showed reduction of mitotic index, appearan…
Cytogenetical test to predict the vigour potential in Brassica seeds under long-term storage.
2013
Preservation of intraspecific diversity in seed banks is a common strategy to frontier the loss of biodi- versity, however long-term storage inevitably results in the loss of a certain percentage of seeds, there- fore causing the genetic erosion of a seed collection. For this reason seed quality monitoring is of out- standing importance to ensure that the regenerated genetic material is representative of the endemic nat- ural diversity once reintroduced in the environment, as well as to preserve the genetic biodiversity of species economically relevant for modern agriculture. Ageing amplification tests, in which seeds under- go osmotic stresses, enable to detect little differences in the vi…